Composite

Part:BBa_K4370010:Design

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-17)
Revision as of 13:36, 18 August 2022 by Sburymone (Talk | contribs) (→‎References)


STREPTO_E.coli_CLONING_MODULE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe).


Source

The sequence has been synthetized.

References

Bai et al., PNAS, 2015 - www.pnas.org/cgi/doi/10.1073/pnas.1511027112

Wang et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00985-13

Aubry et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00485-19