Composite
Part:BBa_K4370010:Design
Designed by: Stéphanie Bury-Moné Group: iGEM22_GO_Paris-Saclay (2022-08-17)
STREPTO_E.coli_CLONING_MODULE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 792
Illegal AgeI site found at 904 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe).
Source
The sequence has been synthetized.
References
Bai et al., PNAS, 2015 - www.pnas.org/cgi/doi/10.1073/pnas.1511027112 Wang et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00985-13 Aubry et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00485-19