Coding
cbbL_STRBO

Part:BBa_K4370000:Design

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-17)
Revision as of 07:14, 22 August 2022 by Sburymone (Talk | contribs) (→‎References)

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cbbL_STRBO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 373


Design Notes

The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli .


Source

This sequence has been synthetized.

References

Reference of Streptomyces bottropensis ATCC 25435 genome: GCF_000383595.1_ASM38359v1