RBS

Part:BBa_K4150000

Designed by: WEI CHIEH CHIN   Group: iGEM22_Mingdao   (2022-08-11)
Revision as of 08:38, 11 August 2022 by Jacky CHIN (Talk | contribs)


g10.RBS

ThisRBS contains a g10 leader sequence from T7 bacteriophage gene 10, which encodes phage coat protein at very high expression level[1].





Reference

  1. ↑ Olins PO, Devine CS, Rangwala SH, Kavka KS. The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli. Gene. 1988 Dec 15;73(1):227-35. doi: 10.1016/0378-1119(88)90329-0. PMID: 3072257.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3


[edit]
Categories
Parameters
None