Part:BBa_K4347005
Backward Loop primer (BL)
The backward loop primer (BL) is one of the six primers needed to carry out Loop Mediated Isothermal Amplification (LAMP).
Usage and Biology
The loop-mediated isothermal amplification (LAMP) is a rapid method that allows for the specific DNA amplification through the utilization of multiple primers and three major steps[1]. This method relies on 3 major components- Bst, a DNA polymerase, and two sets of primers, and the target DNA/RNA. The first step, referred to as the “starting material producing step”, composes the stem-looped DNA that is needed for the second stage. The forward and backward inward and outer primers bind to the complementary sequences within the target DNA sequence[1]. The stem-looped DNA undergoes additional annealing within the target sequences and with the Bst polymerase, which produces stem-loop DNA which concludes the first step[1]. The stem-loop DNA enters into step two- “cycling amplification step”, which uses the internal primers FIP and BIP in hybridization and synthesis reactions to produce an original and a repaired stem-loop DNA strands[1]. For further amplification, loop primers can be inserted into the reactions[2]. The third step is “elongation and recycling”, where the DNA products are recycled and elongated through a BIP-primed strand displacement reaction[1].
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., & Hase, T. (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research, 28(12), e63–e63. https://doi.org/10.1093/nar/28.12.e63
2. Niessen, L., & Vogel, R. F. (2010). Detection of Fusarium graminearum DNA using a loop-mediated isothermal amplification (LAMP) assay. International Journal of Food Microbiology, 140(2–3), 183–191. https://doi.org/10.1016/j.ijfoodmicro.2010.03.036
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