Part:BBa_K4516007

pGL3pro-backbone

contribution

pGL3pro-backbone is a high-copy-number luciferase reporter system carrier, which uses an SV40 promoter to regulate the expression of luciferase genes, and it can be used for rapid and quantitative assessment of factors that affect the expression of specific genes in mammalian cells. When expressed in the prokaryotic system, the Amp+ resistance can be used to screen the right colony. These new composite parts are important for both our project and future projects

Figure 1. The map of three plasmids pGL3pro-3IRE (B)..

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 58
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NotI site found at 2966
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BglII site found at 10
Illegal BamHI site found at 320
Illegal XhoI site found at 6
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 58
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 58
Illegal NgoMIV site found at 73
Illegal NgoMIV site found at 444
Illegal NgoMIV site found at 2513
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1536
Illegal SapI site found at 453