Coding
Sac7e

Part:BBa_K4347006

Designed by: Victor Di Donato, Nicoletta de Maat   Group: iGEM22_Queens_Canada   (2022-07-07)
Revision as of 16:21, 18 July 2022 by Victor5688 (Talk | contribs)

Sac7e

write small introduction about Sac7e here... eg, what is it? what organism does it come from?

Usage and Biology

Write about usage and biology... examples used in previous work and difference between the other ssb protiens such as ss07d (part BBa K3790001)

Sac7e was isolated from Sulfolobus acidocaldarius, however Sso7d is a highly similar homolog from Sulfolobus solfataricus.

These proteins are hyper-thermostable and resistant to pH extremes. Sac7e is thermally stable to 85.5°C.

Compared to other similar proteins, Sac7e showed the highest affinity for dsDNA (KD = 11 μM), with binding sites ~ 6-8 bases per protein.

DNA binding protien "Sac7e" modelled in Pymol.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References


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Categories
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