Composite
Pre A2

Part:BBa_K4060101:Experience

Designed by: YU CHUAN LEE   Group: iGEM21_NYCU-Taipei   (2021-09-30)
Revision as of 14:02, 7 December 2021 by Clairechen (Talk | contribs) (→‎Assembly of Pre A2 (pSB1C3))

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Assembly of Pre A2 (pSB1C3)

The gene fragment Pre A2 is composed of pTetR BBa_R0040 and a double terminator BBa_B0015. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2.

Fig.3 Agarose electrophoresis in order to verify Pre A2. Lane 1, 100bp ladder. Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre A2 digested with EcoRI PstI.

Applications of BBa_K4060101

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