![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Pre B3
Part:BBa_K4060106
Designed by: YU CHUAN LEE Group: iGEM21_NYCU-Taipei (2021-09-30)
Pre B3 gene
a kill switch strategy.
Usage and Biology
The RFP expression of Construct 3 We used RFP(E1010) to monitor the expression of TetR, so that we can figure out how L-arabinose will influence the expression of TetR in our Design 1.
<img src="https://2021.igem.org/File:T--NYCU-Taipei--kilksw_result_1.png" alt="">
<img src="https://2021.igem.org/File:T--NYCU-Taipei--kilksw_result_2.png" alt="">
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 711
Illegal AgeI site found at 823 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |