Coding

Part:BBa_K3738007:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 03:29, 22 October 2021 by Emily Hagens (Talk | contribs) (→‎Design Notes)

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MS2 Bacteriophage Capsid Protein with N-Terminal Polyarginine Tag and C-Terminal Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for use in E.coli.

This part is an improved version of the part BBa_K2683012, and has been modified to a C-terminal Histidine Tag in addition to the N-terminal polyarginine tag. This part has been codon optimized for use in E.coli.

Source

This part comes from the MS2 bacteriophage.

References

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138