Regulatory

Part:BBa_K3844001:Sequence, Features, and Subparts

Designed by: Anya Sabhnani   Group: iGEM21_DNHS_SanDiego_CA   (2021-10-21)
Revision as of 02:24, 22 October 2021 by Anya (Talk | contribs) (→‎3. Characterization)

1. Function

CCCTC-binding factor, otherwise known as (CTCF), is a transcription factor. It has numerous functions in synthetic biology, including but not limited to functioning as a transcriptional activator or an insulator protein. CTCF transcription factors can also call for other transcription factors while bound to these important entities known as “chromatin domain boundaries.”

2. Design Consideration

Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.

3. Characterization

Sequence: GGAGGCCCTCCCACTCTCCTCTCGCACCCGCTTTTAGGTGGCCCTCTTCT AGGGCGGAGAGATCACCAAAAGCCGCTGAGCAAGGACAGCCCTTCCAGTCGTCGCTCGCG GGCACGGAGCTGGGAGCGCGCGGGAACCGACCGGGCCTTACTCTGGGGCGGGCAGCTGT AAGACATTCGGAGGGCGGGTCGGGCCTGGCTGGGGCGGAGACTGGGCGGGGCCGGGCCCG GGAGCCTCCTCAGCCCCACTGGCTTGCCCCGCGCGCGCTTCCTCCAGCCACCCCCACTTC CCCAACCCTGTACGGGTTCAGGGGGCCGGAGACAGCAGCTAGGGAATAAGGTCAAGCGGA CTGGATCCGGCACCGCCCAGGGGCGGCGGCCTCCGCCCAGGAAGCCCCACCCAGCGGGAG CCAGGACGCCGCGGCGCCGGCAGAGCCCACGAGCGACCCGGCGAGGGCGCGTGCGGCGTG ACGACGCGACTCGCGGCGCGTCCCGTGACGCCATCGCGCCCGCTTTTGAAAGTTGGCGCCC GGGGCCGCCTCCCATGCTGCCCTGCGCCAACCCCTCCCCTCACCTTTCCTCCCCCGCCCT CTACTCGCCGCCCGGCCGGTCCCCCCACCCGTCCCTTCCCTTATCAGCACCCGCGGCCCC GGCAGCGCCGACGCAGGCGCACTGCACCGCGCCGCCGCCATTTTGTGTCTGAGCCTGTGGA GCGATTAAACCGTGCGCGGAGCTGCTTCTTTGGCGGCAGCGGCGGCGGCGGTGGCCGGTG CGGACGCGCGGAGCTCGCCGGAGACGCCGGGTGGCCGGAGCCGTGGAGCGGCGGCGGAGCG GGCGCCGCGGGGGGTGTGGCGCGGAG

4. References

1. https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transcription-factor-ctcf 2. Fu Y, Sinha M, Peterson CL, Weng Z (2008). "The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome". PLOS Genetics. 4 (7): e1000138. doi:10.1371/journal.pgen.1000138. PMC 2453330. PMID 18654629. 3. https://pubchem.ncbi.nlm.nih.gov/compound/1_2_4-Trimethylbenzene 4. https://www.researchgate.net/publication/319293199_CCCTC-binding_factor_is_essential_to_the_maintenance_and_quiescence_of_hematopoietic_stem_cells_in_mice