Part:BBa_K3844001:Sequence, Features, and Subparts

1. Function

CCCTC-binding factor, otherwise known as (CTCF), is a transcription factor. It has numerous functions in synthetic biology, including but not limited to functioning as a transcriptional activator or an insulator protein. CTCF transcription factors can also call for other transcription factors while bound to these important entities known as “chromatin domain boundaries.”

2. Design Consideration

Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.

3. Characterization

Sequence: GGAGGCCCTCCCACTCTCCTCTCGCACCCGCTTTTAGGTGGCCCTCTTCTAGGGCGGAGA GATCACCAAAAGCCGCTGAGCAAGGACAGCCCTTCCAGTCGTCGCTCGCGGGCACGGAGC TGGGAGCGCGCGGGAACCGACCGGGCCTTACTCTGGGGCGGGCAGCTGTAAGACATTCGG AGGGCGGGTCGGGCCTGGCTGGGGCGGAGACTGGGCGGGGCCGGGCCCGGGAGCCTCCTC AGCCCCACTGGCTTGCCCCGCGCGCGCTTCCTCCAGCCACCCCCACTTCCCCAACCCTGT ACGGGTTCAGGGGGCCGGAGACAGCAGCTAGGGAATAAGGTCAAGCGGACTGGATCCGGC ACCGCCCAGGGGCGGCGGCCTCCGCCCAGGAAGCCCCACCCAGCGGGAGCCAGGACGCCG CGGCGCCGGCAGAGCCCACGAGCGACCCGGCGAGGGCGCGTGCGGCGTGACGACGCGACT CGCGGCGCGTCCCGTGACGCCATCGCGCCCGCTTTTGAAAGTTGGCGCCCGGGGCCGCCT CCCATGCTGCCCTGCGCCAACCCCTCCCCTCACCTTTCCTCCCCCGCCCTCTACTCGCCG CCCGGCCGGTCCCCCCACCCGTCCCTTCCCTTATCAGCACCCGCGGCCCCGGCAGCGCCG ACGCAGGCGCACTGCACCGCGCCGCCGCCATTTTGTGTCTGAGCCTGTGGAGCGATTAAA CCGTGCGCGGAGCTGCTTCTTTGGCGGCAGCGGCGGCGGCGGTGGCCGGTGCGGACGCGC GGAGCTCGCCGGAGACGCCGGGTGGCCGGAGCCGTGGAGCGGCGGCGGAGCGGGCGCCGC GGGGGGTGTGGCGCGGAG