Composite
Co-op

Part:BBa_K233002:Design

Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil   Group: iGEM09_IBB_Pune   (2009-07-10)
Revision as of 10:26, 18 October 2009 by Swesrini (Talk | contribs) (→‎Design Notes)

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Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3918


Design Notes

A suitable export tag was synthesizd and added in frame. the export tag was designed based on the twin arginine translocation (tat) pathway from E.coli

Source

source- biobricks torA,lacZ, pLac - eschercihia coli GFP- aequoria victoria

References