Part:BBa_K3745000

Prha-Amp

This part is a rhamnose-induced genetic circuit that contains a Rhamnose promoter, an RBS, an ORF of AmpR, and a terminator. It is improved from BBa_K1493501by using Gibson assembly to replace the GFP with AmpR.

Usage and Biology

In our project, it is a vital part of the biosensor. After rhamnolipid is hydrolysis to rhamnose, rhamnose could induce this genetic circuit to express the ampicillin resistance gene. This mechanism increases the evolutionary fitness of the strains that have a high rhamnolipid yield (which means more rhamnose could be hydrolysis and induce the expression of AmpR) in culture with ampicillin and enables the strains with favorable mutations to be automatically selected.

Qualititive Characterization

The following graph demonstrates the validity of this part. With higher rhamnose concentration, the concentration of the bacteria fluid tends to be greater, indicated by a high absorbance at OD600. The absorbance of culture with 0/20/40/60/80ul rhamnose added is measured every hour after liquid inoculation.

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 869