Coding

Part:BBa_K3795000:Design

Designed by: Isis Botelho Nunes da Silva   Group: iGEM21_Rio_UFRJ_Brazil   (2021-10-11)
Revision as of 00:25, 22 October 2021 by Isisbnsilva (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


DME-C: Multi-epitope protein for anti Dengue antibodies detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 276
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is codon-optimized for expression in Escherichia coli. Besides, restriction sites for Biobrick Standard Assembly (EcoRI, PstI, XbaI, SpeI) and also BamHI and NocI were removed.


Source

It was designed by the iGEM 2021 team Rio_UFRJ_Brazil through the combination of promising Dengue epitopes in order to minimize cross-reaction with anti-Zika antibodies. After the design, it was synthesized by Genscript. The initial epitopes were collected from previous work from AnandaRao[1,2], which were submited to bioinformatics analysis in minimize cross-reaction.

References

  1. AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N. A custom-designed recombinant multiepitope protein as a dengue diagnostic reagent. Protein Expression and Purification. 2005;41(1):136–47.
  2. AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N. Recombinant multiepitope protein for early detection of dengue infections. Clinical and Vaccine Immunology. 2006;13(1):59–67.