Coding

Part:BBa_K4088898

Designed by: Ruslan Vasiliev, Yaroslav Ugolkov, Alice Potapova, Pavel Ivanov-Rostovtsev   Group: iGEM21_Moscow_City   (2021-10-21)
Revision as of 00:08, 22 October 2021 by Pavrostiva (Talk | contribs)


dCas13a

Cas13a is a classic RNA-targeting nuclease that is widely used in a variety of diagnostics methods. It is a very specific variant of RNA detection, it can be customized to recognize single nucleotides on the target RNA. dCas13a is a catalytically dead LwaCas13a enzyme.
For more information, see BBa_K4088891.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 622
    Illegal BglII site found at 924
    Illegal BglII site found at 1689
    Illegal BglII site found at 1956
    Illegal BglII site found at 2166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None