Composite

Part:BBa_K3806011:Design

Designed by: Laura Sierra Heras   Group: iGEM21_TUDelft   (2021-09-30)
Revision as of 20:57, 21 October 2021 by LauraSH (Talk | contribs)


Theophylline-binding aptazyme with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence features a T7 promoter for the transcription into a functional aptazyme by the T7 bacteriophage RNA polymerase. The sequence can be amplified by primers BBa_K3806017 and BBa_K3806020.

Source

The sequence of the Theophylline aptazyme was retrieved from the manuscript by Townshed et al. [1].

References

[1] Townshend, B., Xiang, J. S., Manzanarez, G., Hayden, E. J. and Smolke, C. (2021). A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors. Nat Commun, 12, 1437.