RNA

Part:BBa_K3714010

Designed by: Bairun Chen   Group: iGEM21_SJTU-BioX-Shanghai   (2021-09-30)
Revision as of 02:15, 22 October 2021 by Grus (Talk | contribs)


Improved gard-337 for lateral flow assay

To test our optimization design with TMSD, a structureless toehold site between biotin probe binding site and aptazyme domain was need. However, designing the probe with manual work and test with available RNA structure prediction model is such a huge work with plenty of attempts. This part is designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed probe shows no influence on the function of aptazyme, the toehold worked as our expect as well.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


K3714016.png
Figure.1 | Verification of temporarily inhibition and disinhibition by oligonucleotides. The aptazyme was produced by a T7 in vitro transcription system. The product was run on a denatured PAGE. Gardiquimod is an inhibitor of the cleavage activity. "stop" and "start" are two oligonucleotides designed to control the activity of the aptazyme/


[edit]
Categories
//rna/aptazyme
Parameters
None