RNA
Part:BBa_K3714010
Designed by: Bairun Chen Group: iGEM21_SJTU-BioX-Shanghai (2021-09-30)
Improved gard-337 for lateral flow assay
To test our optimization design with TMSD, a structureless toehold site between biotin probe binding site and aptazyme domain was need. However, designing the probe with manual work and test with available RNA structure prediction model is such a huge work with plenty of attempts. This part is designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed probe shows no influence on the function of aptazyme, the toehold worked as our expect as well.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rna/aptazyme
None |