Composite

Part:BBa_K3777029

Designed by: SiYuan Peng   Group: iGEM21_OUC-China   (2021-10-01)
Revision as of 18:03, 21 October 2021 by Wuti (Talk | contribs)


luxR-Plux-dCas9-mphA

A biosensor device that can improve the detection limit of erythromycin.

Usage and Biology
Compared to our another part BBa_K3777028,this part add a gene called mphA, which can improve the detection limit of erythromycin
According to the paper we reference,it is said that trapping erythromycin within Escherichia coli through phosphorylation increases the sensitivity of its biosensor (MphR) by approximately 10-fold. 798px-LuxR-Plux-dCas9-mphA.PNG

Referernce:Miller Corwin A,Ho Joanne M,Parks Sydney E,Bennett Matthew R. Macrolide Biosensor Optimization through Cellular Substrate Sequestration.[J]. ACS synthetic biology,2021,10(2)
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2204
    Illegal NheI site found at 5364
    Illegal NheI site found at 5387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1022


[edit]
Categories
Parameters
None