Composite

Part:BBa_K3777029

Designed by: SiYuan Peng   Group: iGEM21_OUC-China   (2021-10-01)
Revision as of 18:01, 21 October 2021 by Wuti (Talk | contribs)


luxR-Plux-dCas9-mphA

A biosensor device that can improve the detection limit of erythromycin.

Usage and Biology
Compared to our another part BBa_K3777028,this part add a gene called mphA, which can improve the detection limit of erythromycin 798px-LuxR-Plux-dCas9-mphA.PNG

Referernce:Miller Corwin A,Ho Joanne M,Parks Sydney E,Bennett Matthew R. Macrolide Biosensor Optimization through Cellular Substrate Sequestration.[J]. ACS synthetic biology,2021,10(2)
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2204
    Illegal NheI site found at 5364
    Illegal NheI site found at 5387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5951
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1022


[edit]
Categories
Parameters
None