Composite

Part:BBa_K4059030:Design

Designed by: Ann-Sofie Nielsen   Group: iGEM21_SDU-Denmark   (2021-10-21)
Revision as of 22:42, 21 October 2021 by AnnSofie1 (Talk | contribs) (→‎Design Notes)

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CPR from Phanerochaete chrysosporium with N-terminal deletion (12), E. coli K12 codon optimized


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2336
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2337
    Illegal PstI site found at 2351
    Illegal NotI site found at 152
    Illegal NotI site found at 2344
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2337
    Illegal PstI site found at 2351
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Deletion of CPR

Source

.

References