Regulatory

Part:BBa_K3905000

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-04)
Revision as of 13:33, 21 October 2021 by PHeywood (Talk | contribs)


gen1 517-5p Toehold Switch

Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None