Part:BBa_K4011006

AeAT9

AeAT9 is an alcohol acyltransferase, and is responsible for the production of ethyl-acetate from acetyl-CoA and ethanol in our fermentation process. We will express AeAT9 in yeast cells to synthesize ethyl-acetate from acetyl-CoA and ethanol, the second step into our pathway to turn acetate and ethanol into ethyl-acetate. We hope to give our yeast culture a fruit smell. This part will also be used to construct yeast expression plasmids through golden gate assembly into BBa_K4011017 and BBa_K4011018.

Usage and Biology

AeAT9 is an alcohol acyltransferase from Actinidia eriantha, otherwise known as kiwifruit. Its general function is to convert alcohols into esters. The fruity smell one smells from kiwifruit it largely a result of AeAT9.

It was first characterized and expressed in yeast to produce ethyl-acetate by Shi et al in 2021.

Source

Source: AeAT9 is from Actinidia eriantha.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 199
  Illegal BglII site found at 817
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 226
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1064