Part:BBa_K4011001
CBM2
CBMs are artificial proteins derived from natural proteins with cellulose-binding functions, such as cellulase. There are three types of CBMs, which are CBMs, CBM1, CBM2, and CBM3. CBM1 is the smallest, whilst CBM3 is the biggest. By fusing CBMs to functionalization proteins, we can achieve modification/functionalization of our bacterial cellulose membrane. This is part in a part collection where we characterize bacterial cellulose modification methods and constructs using CBMs.
The part collection includes: Cellulose binding matrix BBa_K4011000 and BBa_K4011001. CBMs fused with spider silk fibroin BBa_K4011008 and BBa_K4011009. Fused proteins capable of expression and secretion in S. cerevisiae BBa_K4011010 and BBa_K4011011.
This part collection can help and inspire other teams we are trying to achieve modification of cellulose membranes using different modification/functionalization proteins.
Usage and Biology
In nature, CBM2s are expressed as a domain of a protein whose functions require being bound to cellulose, such as cellulase. The structure of CBM2 is displayed in the Characterization section (Protein Data Bank accession: 1EXG).
CBM2 fused with spider silk proteins is first done by Mohammadi et al in 2019, where they tested the changes in physical properties on cellulose fibers after mixing with CBM2-spider silk.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 52
Illegal AgeI site found at 96
Illegal AgeI site found at 268
Illegal AgeI site found at 334 - 1000COMPATIBLE WITH RFC[1000]
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