Composite

Part:BBa_K4046780

Designed by: Jessica Shah   Group: iGEM21_Duke   (2021-10-19)
Revision as of 15:16, 21 October 2021 by Shahjessica (Talk | contribs)


CMV - BS #2 - BS #2 - BS #2 - Kozak - tdTomato - bghA

This is a binding site for the DhdR gene, as identified in the literature by Xiao et al. In this composite part, we added a Kozak sequence and tdTomato gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG. For more information about the expected behavior of the circuit, please consult the “Design Considerations section. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1040
    Illegal PstI site found at 1766
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1040
    Illegal PstI site found at 1766
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1040
    Illegal PstI site found at 1766
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1040
    Illegal PstI site found at 1766
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None