Translational_Unit

Part:BBa_K3765017

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)
Revision as of 19:06, 20 October 2021 by Dipayan akhuli (Talk | contribs)

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T7 RBS + OpdA+SpyTag002+sfGFP with OpdA signal peptide

Translational Unit comprising of the CDS of an OpdA-SpyTag002-sfGFP fusion protein under a strong RBS from bacteriophage T7 gene 10.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1252
    Illegal AgeI site found at 1315
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/degradation
//function/reporter/fluorescence
Parameters
None