Composite

Part:BBa_K3739059

Designed by: Ziyan Han   Group: iGEM21_XMU-China   (2021-09-09)
Revision as of 17:50, 20 October 2021 by Ettrium (Talk | contribs)


J23100-B0030-pelB-Ag43-160N-Tevp-B0010

Tevp can cut Ag43, leaving pelB as a signal peptide

Usage and Biology

Ag43 is a surface display protein that displays protein molecule it carries on the cell membrane of bacteria. pelB is an exopeptide linked to Ag43. Tevp can shear Ag43 and release the exopeptide pelB to secrete the protein molecules out of bacteria.

Reference

1. Ahan, R. E.; Kirpat, B. M.; Saltepe, B.; Seker, U. O. S., A Self-Actuated Cellular Protein Delivery Machine. ACS Synth Biol 2019, 8 (4), 686-696.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 118
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 2180
    Illegal NgoMIV site found at 2690
    Illegal NgoMIV site found at 2711
    Illegal AgeI site found at 1706
    Illegal AgeI site found at 2450
    Illegal AgeI site found at 2864
    Illegal AgeI site found at 3106
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None