Coding

Part:BBa_K3739016

Designed by: Han Ziyan   Group: iGEM21_XMU-China   (2021-09-08)
Revision as of 19:16, 21 October 2021 by XPIG (Talk | contribs)


AKTA-his-Mfp5

It is a kind of mucin from mussels, including AKTA expression tags and 6xHis affinity tags which can let the mucin purified by tags.

Biology

Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It’s side chain contains a lot of dopamine and is the main factor of adhesion.
Fig.1. Mfp5 adhesion mechanism

Usage

By codon optimization and adding a 6His-tag, the sequence suitable for expression in BL21(DE3) was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter to obtain BBa_, and the constructed plasmid was transformed into BL21(DE3) to verify its expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None