Coding

Part:BBa_K3863004

Designed by: LEONG WENG I   Group: iGEM21_PuiChing_Macau   (2021-09-26)
Revision as of 16:54, 20 October 2021 by Alisaleong (Talk | contribs)


Phac-flag-PCT(optimized)-HA-his

This is a composite part designed to produce bioplastic PHA with his-tag. PHA synthase 1 from Pseudomonas resinovorans. The protein sequence in this part is based on the K2042001 sequence Evry 2016. PCT is propionate CoA transferase from Clostridum propionicum. The protein sequence in this part is based on the K1211001 sequence Yale 2013. Tackling the issues with biobricks, we have optimized the codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
    Illegal BamHI site found at 1506
    Illegal XhoI site found at 1030
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 2125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 251



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Categories
Parameters
None