Composite
Part:BBa_K3747003:Experience
Designed by: Riemer van der Vliet Group: iGEM21_Wageningen_UR (2021-10-20)
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how you used this part and how it worked out.
Applications of BBa_K3747003
SDS-page of particulate methane monooxygenase and scaffold. pMMO mimic as described in Kim et al 2019, expressed in E. coli BL21 strain with pET-28(+)-pMMO-m1 plasmid. The lanes harbor left to right (1) PageRuler Prestained Protein Ladder product #26616. (2) Product after Sonication. (3) Product after filtered. (4) Product after His-tag purification. The expected product size is 54 kDA in all cases and is annotated with the white asterisk in the figure.
User Reviews
UNIQ651cc309054c3573-partinfo-00000000-QINU UNIQ651cc309054c3573-partinfo-00000001-QINU