Part:BBa_K3886026
L-arabinose-inducible Chromoprotein eforRed expression circuits
This composite part is used to achieve L-arabinose-induced Chromoprotein eforRed expression. We tested this circuit in our Hidro system. To learn more about Hidro system, please visit NDNF_China Proof-Of-Concept.
Characterization
To determine whether encapsulated bacteria in Hidro can respond to these external signal inputs, we encapsulated Hidro with the bacteria containing a genetic circuit that expresses chromoprotein (eforRed) in response to L-arabinose induction (Figure 1A). We then incubated the beads at 37 °C in the absence of L-arabinose or in the presence of 10mM L-arabinose. We found that encapsulated cells exposed to L-arabinose exhibited a pink colour compared with encapsulated cells not exposed to L-arabinose (Figure 1B). Thus, gene expression in Hidro can be exogenously controlled by chemical inducers.
Figure 1: (A) The schematic of a L-arabinose–controlled genetic switch to produce chromoprotein eforRed; (B) eforRed protein production in Hidro.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1214
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1474
Illegal AgeI site found at 1049 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1031
| None |