Coding
amyRFP

Part:BBa_K4032003

Designed by: Tsugumi Ohzeki   Group: iGEM21_Gunma   (2021-09-30)
Revision as of 05:56, 21 October 2021 by Tsubasa O (Talk | contribs)


amylase-RFP


This part contains the gene of the amylase-RFP fusion protein.

See NCBI: NC_000913.3for information on the amylase gene malS.

Usage and Biology

This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. See NCBI: NC_000913.3for details.

In the case of this part, red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.


This part expresses the fusion protein of amylase (from BBa_K523006) and RFP (from BBa_J04450). We removed the stop codon of the amylase gene and the start codon of the RFP gene and connected the two ends.


The RBS is added to this part, forming BBa_K4032006.

The lac promoter, the RBS and the double terminator are added to this part, forming BBa_K4032104.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 336
    Illegal AgeI site found at 1501
    Illegal AgeI site found at 2580
    Illegal AgeI site found at 2692
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None