Coding

Part:BBa_K3888003

Designed by: Linhan Lei   Group: iGEM21_SJTang   (2021-10-01)
Revision as of 11:57, 20 October 2021 by RyanLei (Talk | contribs)


HoxG1

HoxG1 coding gene with E. coli signal sequence
This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli. HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with HoxK1(BBa_K3888004) to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).

PET28-pTac-lacO-hydrogenase .png

Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.

HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator.


Sequence and Features

HoxK1 has a length of 966bp, which is located from 7137bp to 8102bp on the plasmid diagram showed above.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 282
    Illegal XbaI site found at 384
    Illegal SpeI site found at 450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 450
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 597
    Illegal BamHI site found at 1512
    Illegal XhoI site found at 1834
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 282
    Illegal XbaI site found at 384
    Illegal SpeI site found at 450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 282
    Illegal XbaI site found at 384
    Illegal SpeI site found at 450
    Illegal AgeI site found at 1728
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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