Generator

Part:BBa_K3792006

Designed by: Chase Tarillo   Group: iGEM21_FSU   (2021-09-24)
Revision as of 00:45, 20 October 2021 by Anishshahez (Talk | contribs)


NodCIJ Generator I

The NodCIJ Generator with Weak Promoter does not produce a lot of the NodC protein, NodI protein, and NodJ protein. The NodC protein produces chitin and NodI and NodJ transports nodulation factors.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 854

This part consists of 2555 base-pair length. It includes a promoter, 3 ribosome binding sites, 3 protein-coding sequences, and a terminator. We used promoter K2832184, a weak promoter from the BIOFAB collection. In addition, we also used protein-coding sequences from Chitin synthase from Rhiozobium leguminosarum, Nodl a protein possibly involved in transporting chitin across the cell membrane, and NodJ, a protein also possibly involved in the transportation of chitin



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Categories
Parameters
None