Part:BBa_K3286138
flg22
Flg22 is a peptide consisting of 22 conserved amino acids at the N-terminus flaggelin of Pseudomonas syringae [Felix et al., 1999]. Flg22 is a well described Microbe Associated Molecular Pattern (MAMPs). If recognised, MAMPS trigger PAMP Triggered Immunity in plants. They have been shown to trigger disease resistance in plants. [Zipfel et al., 2004]. flg22 can be used to active an immune response in plants.
It is very hard to purify this peptide if recombinantly expressed in E. coli, due to the small size and the folding of this peptide.
Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x Felix, G., Duran, J. D., Volko, S., & Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. The Plant Journal : For Cell and Molecular Biology, 18(3), 265–276. https://doi.org/10.1046/j.1365-313x.1999.00265.x
Characterization by team 2021 UZurich
Seedling growth inhibition (SGI) assay was performed to characterize the immunogenicity of flg22 and elf18 peptide (by chemical synthesis).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 58
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 58
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 58
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 58
Illegal NgoMIV site found at 52
Illegal AgeI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
| None |