Coding

Part:BBa_K3889025

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-02)
Revision as of 06:38, 20 October 2021 by AshWinShaRma (Talk | contribs)


Bacillus subtilis yqcG toxin

YqcG toxin is a part of type 2 toxin-antitoxin system namely YqcGF from Bacillus subtilis [1]. YqcF (BBa_K3889025) binds to YqcG in order to inhibit the toxin. YqcG is a DNase that can lead to DNA degradation finally causing death due to loss of genetic material.

This is a modified version specific of BBa_K3507002 to our project's assembly. Xho1, HindIII and Bsa1 sites were mutated to make an assembly compatible part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Brantl, S., & Müller, P. (2019). Toxin–Antitoxin Systems in Bacillus subtilis. In Toxins (Vol. 11, Issue 5, p. 262). MDPI AG. https://doi.org/10.3390/toxins11050262

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