Part:BBa_K3794001

PVFP-5 Composite This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein, BBa_K3794000, in E.coli. It is under the control of the lac promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021. Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column. This composite part / translational unit does not contain a terminator, therefore a plasmid, such as pSB1A3, containing a terminator region or an addition terminator part from the iGEM registry is required. ===Usage and Biology=== Sequence and Features BBa_K3794001 SequenceAndFeatures