Part:BBa_K4015002
DsbA
DsbA is a soluble periplasmic protein. It belongs to the thioredoxin family and acts as a type of signal peptide which serve as the primary source of disulfide bonds to secreted proteins. The protein catalyzes the oxidation of the two cysteine sulfhydryls and form a covalent S-S bond connecting amino acid, then donates to the secreted protein, catalyzing their oxidation and their folding. DsbA’s size is about 21-kDa, primarily active in type II secretion peptide.
Usage and Biology
In our project, we pick three signal peptides to help secrete our target proteins, which are HlyA、GeneIII and DsbA . We used p47 vector containing GFP to compare the effectiveness of these signal peptides. p47-GFP-HlyA displayed a lower level of fluorescence comparing to p47-GeneIII-GFP, suggesting HlyA is more effective in secretion since more GFP has entered the medium. p47-DsbA-GFP showed no fluorescence, meaning protein expression was inhibited or the folding of the protein has failed.
Fig. Result of GFP secretion experiment
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |