Plasmid

Part:BBa_K3765011

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)
Revision as of 07:05, 21 October 2021 by Dipayan akhuli (Talk | contribs)

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pETMCN-T7

High copy number KanR vector which can be used for expression under a strong T7 promoter. The promoter, operator, RBS and terminator are already included in the plasmid, so any CDS can be cloned into the MCS and be ready to express.

Usage and Biology

This vector was used in our project to clone and express all of our constructs coding for fusion proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5111
    Illegal SpeI site found at 5030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5093
    Illegal XhoI site found at 5075
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


[edit]
Categories
//plasmid
//plasmid/expression/t7
Parameters
None