Composite

Part:BBa_K3740042

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-09-22)
Revision as of 06:49, 19 October 2021 by HUANG (Talk | contribs) (→‎2021 SZPT-China)


Chimeric Bacteriocin SE

Description

We replaced the nuclease domain of pyocin S2 with that of colicin E3 to produce a chimeric bacteriocin termed SE.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2407
    Illegal BglII site found at 2112
    Illegal BamHI site found at 1684
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2407
    Illegal NgoMIV site found at 1483
    Illegal AgeI site found at 1573
  • 1000
    COMPATIBLE WITH RFC[1000]


2021 SZPT-China

Biology

The receptor domain, translocase domain of pyocin S2 and the nuclease domain of colicin E3 are fused together to get a new chimeric bacteriocin termed SE. The antipseudomonal SE protein can target and inhibit P. aeruginosa, even including those harbouring a S2 immunity protein IMMS2

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Categories
Parameters
None