Composite

Part:BBa_K3827002:Design

Designed by: Livia Vieira   Group: iGEM21_British_Columbia   (2021-10-18)
Revision as of 16:58, 20 October 2021 by Livialopes (Talk | contribs)

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Lactate Inducible Reporter (pLactate + lldR + RFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1018
    Illegal NheI site found at 1041
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 773
    Illegal AgeI site found at 885
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to ensure that each part was compatible to be able to be used in either E. coli or Salmonella. Experiments were run to determine the activity of the lactate regulatory unit and RFP fluorescence was measured against OD600.


Source

Salmonella enterica strain SP chromosome, complete genome (NCBI) [for pLactate and lldR]

Biobrick parts [for constitutive promoter, RBS, RFP, and terminator]

References