Part:BBa_K3805138:Design

agrBD

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

For this part, we design two validation experiments

experiment2：Measurement of AIP generation

According to our pathway design (below), specific binding of AIP to a protein on the guard membrane activates the activity of this membrane protein, AgrC, which drives AgrD phosphorylation and activates the P2 promoter, thereby driving the release of mcherry to the extracellular compartment. Therefore, if you want to test the concentration of AIP, you need to measure the amount of mcherry

File:Pathway.jpeg

our pathway design

The experimental design was divided into two steps. Step 1: Obtaining standard data for AIP Add a specified amount of AIP solution to the guard's bacterial solution and measure the amount of mcherry Step 2: Determine the amount of mcherry in the deceiver and fit the actual process to the deceiver AIP yield based on the standard data

Source

This part contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC4137.

References

[1] Novick RP, Geisinger E. Quorum sensing in staphylococci. Annu Rev Genet. 2008;42:541-64. [2] Liang H, Deng X, Bosscher M, Ji Q, Jensen MP, He C. Engineering bacterial two-component system PmrA/PmrB to sense lanthanide ions. J Am Chem Soc. 2013 Feb 13;135(6):2037-9. [3] Gardner TS, Cantor CR, Collins JJ. Construction of a genetic toggle switch in Escherichia coli. Nature. 2000 Jan 20;403(6767):339-42.