Tag
MFa1-Secre

Part:BBa_K792002:Experience

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-24)
Revision as of 04:44, 18 October 2021 by CWChew (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K792002

User Reviews

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manugimenez

Successfully used in BBa_K792010, to secrete a Trp-riched peptide. Check BBa_K792010 entry to get more information about the experience.

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CWChew

Successfully expressed GFP using this part to quantify the performance. Characterization of part performance was also done using a GFP reporter.

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Figure 1: Image of the media supernatant, resuspended cells and blank control media under gel visualizer blue light, media and supernatant are clearly fluorescent compared to the control media.

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Figure 2: mFa-GFP construct assembled to test the performance of this secretion tag.

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Figure 3: GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741), after normalization to blank media. Induced cells secreted approximately 42% of the GFP, while uninduced cells produced and secreted significantly less GFP than induced cells.


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