Regulatory

Part:BBa_K3875019

Designed by: Yimiao Lin   Group: iGEM21_BUCT   (2021-10-01)
Revision as of 12:23, 9 October 2023 by Conerrr (Talk | contribs) (→‎Usage and Biology)

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Usage and Biology

It is a bacterial promoter (Registry of Standard Biological Parts BBa_J23119) that modified to end with an SpeI site. J23119 is the "consensus" promoter sequence and the strongest member of the family. The sgRNA will transcribe from the J23119(SpeI) promoter.


iGEM23_SCUT-China

In 2023, SCUT-China successfully applied the CRISPRi system containing this promoter in a novel strain of Streptomyces sp. SCUT-3 and tested effective. Details of the usage can be found in BBa_K4621080.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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