Plasmid
pGEM-SBPas

Part:BBa_K4047039:Experience

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-06)
Revision as of 17:58, 20 October 2021 by Imesam (Talk | contribs)

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This plasmid is for deletion of SBPase, and was successfully transformed into Synechococcus elongatus cells. Using segregation PCR, we confirmed that only few copies of the genome could contain the inactivated SBPase with affecting cell viability, but the plasmid was able to successfully integrate the selective marker for kanamycin resistance into the target gene.

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UNIQ79863c81ce248b8e-partinfo-00000000-QINU UNIQ79863c81ce248b8e-partinfo-00000001-QINU