Part:BBa_K3733037:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3733037

We transform E.coli DH5α strain with BBa_K3733037 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM, 10mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 50 and OD₆₀₀ in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

Figure 1.The curve of OD₆₀₀ corrected RFU values change with time under induction of different concentrations of thiosulfate

User Reviews

UNIQ46b3f4b8c44aa21f-partinfo-00000001-QINU UNIQ46b3f4b8c44aa21f-partinfo-00000002-QINU