panS-loxP-MCS
This shuttle vector was modified based on the shuttle vector BBa_K3228089 designed by iGEM Team Marburg 2019. It contains the ori ColE1 for E.coli and the minimal replication region of the pANS plasmid for S.elongatus. The minimal replication region of the pANS plasmid has been codon optimized for S.elongatus UTEX 2973 (see BBa_K3776007)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2378
Illegal AgeI site found at 4103 - 1000COMPATIBLE WITH RFC[1000]