Reporter

Part:BBa_J70029:Design

Designed by: Tom Knight   Group: Knight Lab   (2009-03-27)
Revision as of 20:46, 18 June 2009 by Tk (Talk | contribs) (→‎Source)

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RBS + mCherry gene, optimized for expression in Me. florum and E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Initial tag of mCherry replaced with original AA sequence to eliminate the cryptic RBS + start internal to the coding sequence. Removed PvuII and Sau3AI sequences. Use of the compromise codon table guarantees no CGG codons and the use of TGG for tryptophan.


Source

Synthesized by DNA 2.0, designed with Gene Designer. Initial tag of mCherry replaced with original AA sequence to eliminate the cryptic RBS + start internal to the coding sequence. Removed PvuII and Sau3AI sequences. Use of the compromise codon table guarantees no CGG codons and the use of TGG for tryptophan.

The part is available in the DNA 2.0 pJ201 plasmid, which is a pUC19 style plasmid, Kan resistant.

References