Coding

Part:BBa_K3989010

Designed by: Tianyu Xu   Group: iGEM21_UZurich   (2021-09-30)
Revision as of 15:52, 16 October 2021 by ZhangSongyuan (Talk | contribs)


plant immune elicitor flg22 fused with ClyA for OMV display

In this construct, outer membrane-associated protein ClyA is used to display the plant immune elicitor flg22 to trigger plant immune response.

ClyA in omp8 OMV.jpeg

Omp8 OMVs on col-0.jpeg

TolB OMVs on col-0.jpeg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1105
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1105
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1105
    Illegal NgoMIV site found at 1099
    Illegal AgeI site found at 1063
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None