![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
davBA-AK
Part:BBa_K3776006
Designed by: Helene Heling Huang Group: iGEM21_HK_SSC (2021-10-01)
Revision as of 13:32, 17 October 2021 by Meowstarperson (Talk | contribs)
davB-davA-AK
This part allows overproduction of lysine by a feedback insensitive AK and conversion of L-lysine to 5-aminovaleramide (5AVA) by DavB and DavA in Synechococcus elongatus UTEX 2973. Promoters Pcpc560, 6803PsbA2 and terminator TrbcL are from the genome Synechocystis sp. PCC6803.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 800
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1889
Illegal BglII site found at 4844 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2049
Illegal NgoMIV site found at 3816
Illegal AgeI site found at 1456
Illegal AgeI site found at 1705
Illegal AgeI site found at 3040
Illegal AgeI site found at 5043 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |